The phytochemical screening and antioxidant potential of bark and stem of Parthenocissus quinquefolia (L.) planch was assessed in order to verify its ethnopharmacological significance. All major secondary metabolites e.g. alkaloids, flavonoids, saponins, terpenoids, tannins, reducing sugars, cardiac glycosides and anthraquinones were present. Antioxidant activity was analysed by using five techniques which included DPPH Free Radical Scavenging Activity, FRAP (Ferric Reducing Antioxidant Power), TAA (Total Antioxidant Activity, TPC (Total Phenolic Content and MC (Metal Chelating) Activity. Ethanolic extract of bark showed the highest scavenging effects of 90.01±0.01%, with IC50 value of 24.32mg/ml. Aqueous stem extract showed best activity with IC50 value of 13.6±0.34mg/ml. The significance antioxidant potential indicates the effectiveness of bark and stem of P. quinquefolia in treatment of many diseases.