Physiological and immunological responses to mass mortality in noble scallop Chlamys nobilis cultured in Nan'ao waters of Shantou, China

Abstract

The noble scallop Chlamys nobilis has been a commercially important marine cultured bivalve in the Southern Sea of China for decades. Mass mortality events, however, often occur during scallops' cultivation. Mortality of up to 67%-90% was recorded at the beginning of March in 2017 in some culture areas of Nan'ao (Shantou, China), spreading to all scallops within a week. In the present study, in order to investigate the response of the noble scallop at the physiological and molecular level during mass mortality, scallops with different mortalities of 90%, 67%, and 6% were sampled from three sites at Hunter bay, Baisha bay, and Longhai, respectively. Total carotenoids content (TCC), total antioxidant capacity (TAC), malondialdehyde (MDA) content and the expression levels of three immune-related genes (toll-like receptor, C-type lectin receptor and big defensing) in different scallop tissues were determined. The scallops were divided into three groups of sub-health, lesion, and health. TAC, TCC, as well as transcript levels of CnTLR-1, Cnlec-1 and CnBD in sub-health and lesion scallops were all significantly lower (P < 0.05) than those in health scallops, while MDA in sub-health and lesion scallops were significantly higher than those in health scallops (P < 0.05). Similarly, TCC and TAC in lesion scallops were both higher than sub-health scallops. Moreover, significantly positive correlations were found between TCC and TAC (P < 0.05) and between CnTLR-1 and Cnlec-1 (P < 0.05), while significantly negative correlations were found between TCC and CnTLR-1 (P < 0.05), TCC and Cnlec-1 (P < 0.05), TAC and CnBD (P < 0.05), as well as between MDA and Cnlec-1 (P < 0.001). All the results indicate that noble scallops significantly change their physiological and molecular levels when suffering from stress, and that their antioxidant and immune response systems play important defense functions.

Keywords: Big defensin; C-type lectin receptor; Carotenoid; Chlamys nobilis; Immune response; Mass mortality; Noble scallop; Toll-like receptor.