The present work investigated the antioxidative, anti-inflammatory and organ protective effects of residue polysaccharide (RPS) and its enzymatic-RPS (ERPS) of Lentinula edodes against the LPS-induced sepsis in mice. The results demonstrated that ERPS showed superior effects on inhibiting serum activities of CKMB, LDH, AST, ALP and ALT, lowing the serum levels of BUN, CRE and UA, as well as improving the antioxidant status (SOD, CAT, GSH-PX and T-AOC) than RPS. Moreover, the levels of TNF-α (667.17 ± 36.72 pg/mL), IL-6 (288.47 ± 16.48 pg/mL), and IL-1β (61.62 ± 3.54 pg/mL) on ERPS-treated mice (600 mg/kg bw) significantly lower than that in the LPS-induced mice. Furthermore, the histological observations also indicated the significant recovery effect on organs. The RPS yield was 5.03%, and the ERPS yield was 73.65%. Moreover, the physical properties of polysaccharides were investigated by HPGPC, FT-IR, NMR, GC-MS and AFM analysis, and the results indicated that ERPS was a homogeneous heteropolysaccharide by (1 → 6)- and (1 → 3)-linkages. These conclusions suggested that ERPS might be suitable for functional foods and potentially effective candidate medicine for the treatment of sepsis and its complications.
Keywords: Anti-inflammatory; Antioxidant; Lentinula edodes residue polysaccharide.
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