Proanthocyanidins (PAs) are widespread oligomeric and polymeric flavan-3-ols with significant benefits to human and animal health. As products of the general flavonoid pathway, the biosynthesis of the flavan-3-ols is well-understood and the major enzyme-encoding genes that determine PA structure have been identified. However, the mechanism of PA polymerization remains unknown. The most important transcription factors regulating PA biosynthesis are the MYB factors, potent tools for enhancing PA biosynthesis in plants. In some species, simple overexpression of these transcription factors has led to spectacular successes in upregulating PA synthesis. However, targeted metabolic engineering of the PA structure has not yet been achieved.
Keywords: biosynthesis; crop plants; flavan-3-ols; genetic engineering; proanthocyanidin.