Extruded sorghum (Sorghum bicolor L.) reduces metabolic risk of hepatic steatosis in obese rats consuming a high fat diet

Food Res Int. 2018 Oct:112:48-55. doi: 10.1016/j.foodres.2018.06.004. Epub 2018 Jun 4.

Abstract

The study investigated the effect of extruded sorghum flour (ESF) in a high fat diet (HFD) on biometric measurements and hepatic lipogenesis. Male Wistar rats were fed a normal diet (AIN-93M), HFD, HFD plus ESF replacing 50% cellulose and 100% corn starch (HFDS50), or HFD plus ESF replacing 100% cellulose and 100% corn starch (HFDS100) for eight weeks. ESF reduced the body mass index and liver weight of obese rats. Additionally, ESF reduced hepatic lipogenesis by increasing adiponectin 2 receptor gene expression and gene and protein expressions of peroxisome proliferator-activated receptor α (PPARα), while reducing the gene expression of sterol regulatory element-binding transcription factor 1. Molecular docking analysis revealed the affinity of ESF compounds (luteolinidin, apigeninidin, 5-methoxy-luteolinidin, and 7-methoxy-apigeninidin) with the PPAR-α receptor. Histological analysis confirmed the decreased grade of hepatic steatosis in obese rats. These data indicate the potential of ESF to reduce metabolic risk of hepatic steatosis associated with lipogenesis and obesity.

Keywords: Adipogenese; Anthocyanin; Bioactive compounds; Sorghum; Tannins; β-Oxidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed*
  • Animals
  • Diet, High-Fat*
  • Disease Models, Animal
  • Fatty Liver / genetics
  • Fatty Liver / metabolism
  • Fatty Liver / pathology
  • Fatty Liver / prevention & control*
  • Flour*
  • Gene Expression Regulation
  • Lipogenesis*
  • Liver / metabolism*
  • Liver / pathology
  • Male
  • Molecular Docking Simulation
  • Obesity / diet therapy*
  • Obesity / genetics
  • Obesity / metabolism
  • Obesity / pathology
  • Organ Size
  • PPAR alpha / genetics
  • PPAR alpha / metabolism
  • Rats, Wistar
  • Receptors, Adiponectin / genetics
  • Receptors, Adiponectin / metabolism
  • Sorghum*
  • Sterol Regulatory Element Binding Protein 1 / genetics
  • Sterol Regulatory Element Binding Protein 1 / metabolism
  • Time Factors
  • Weight Loss

Substances

  • PPAR alpha
  • Receptors, Adiponectin
  • Srebf1 protein, rat
  • Sterol Regulatory Element Binding Protein 1
  • adiponectin receptor 2, rat