Identification and verification of differentially expressed microRNAs and their target genes for the diagnosis of esophageal cancer

Xiaoyue Cai; Xiwen Yang; Changjuan Jin; Lei Li; Qing Cui; Yijun Guo; Yun Dong; Xiaohua Yang; Lili Guo; Ming Zhang

doi:10.3892/ol.2018.9066

Identification and verification of differentially expressed microRNAs and their target genes for the diagnosis of esophageal cancer

Oncol Lett. 2018 Sep;16(3):3642-3650. doi: 10.3892/ol.2018.9066. Epub 2018 Jul 4.

Authors

Xiaoyue Cai¹, Xiwen Yang¹, Changjuan Jin¹, Lei Li¹, Qing Cui¹, Yijun Guo¹, Yun Dong¹, Xiaohua Yang², Lili Guo², Ming Zhang¹

Affiliations

¹ Department of Integrated Traditional Chinese Medicine and Western Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, P.R. China.
² Research Center, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, P.R. China.

Abstract

Bioinformatic tools were used to analyze GSE6188, GSE13937 and GSE43732 microarrays, and the top 10 upregulated and downregulated genes of each microarray were identified. It was determined that human microRNA (hsa-miR)-1 and hsa-miR-203 were two downregulated genes in common. Subsequently, it was identified that there were 145 and 335 genes in common targeted by hsa-miR-1 and hsa-miR-203, respectively. In order to narrow the number of target genes down further, the target genes were compared with GSE26886 microarray data. There were five upregulated genes in common with hsa-miR-1, i.e., MMD, BICD1, PTPRG, SDC2 and SEMA6D, and there were eight upregulated genes in common with hsa-miR-203, i.e., PXDN, NRCAM, FMNL2, EIF5A2, GLI3, FSL1, GREM1 and AHR. These genes may become promising biomarkers for the diagnosis of esophageal cancer.

Keywords: biomarker; esophageal cancer; hsa-miR-1; hsa-miR-203.