Long non-coding RNA H19 has been identified to be dysregulated in a number of tumor types, and is closely associated with cancer progression. RAS/mitogen-activated protein kinase (MAPK) is an important intracellular signaling transduction pathway. Activation of the RAS-MAPK signaling pathway is one of the most frequent carcinogenic events in human cancer. However, the mechanism of H19 in promoting the migration and invasion of colorectal cancer (CRC) cells, and the association between H19 and RAS-MAPK signaling pathway is not well understood. The aim of the present study was to investigate the function of H19 on CRC metastasis and invasion, and assess the association between H19 and the RAS-MAPK signaling pathway. The migration and invasion of CRC cells were analyzed using Transwell migration and invasion assays. To elucidate the association between H19 and the RAS-MAPK signaling pathway and determine the expression level of active RAS in CRC cells, Ras activity assay and Western blotting were performed. It was indicated that the overexpression of H19 was able to increase the migration and invasion of CRC cells and this may be mediated by the regulation of RAS activation. Therefore, H19 may promote metastasis and invasion in colorectal cancer by activating the RAS-MAPK signaling pathway.
Keywords: H19; RAS-mitogen-activated protein kinase; colorectal cancer; invasion; migration.