Anti-inflammatory and cytotoxic withanolides from Physalis minima

Jiangping Wu; Xia Li; Jianping Zhao; Ruijuan Wang; Zifei Xia; Xiaoran Li; Yanli Liu; Qiongming Xu; Ikhlas A Khan; Shilin Yang

doi:10.1016/j.phytochem.2018.08.009

Anti-inflammatory and cytotoxic withanolides from Physalis minima

Phytochemistry. 2018 Nov:155:164-170. doi: 10.1016/j.phytochem.2018.08.009. Epub 2018 Aug 17.

Authors

Jiangping Wu¹, Xia Li², Jianping Zhao³, Ruijuan Wang⁴, Zifei Xia⁵, Xiaoran Li⁵, Yanli Liu⁶, Qiongming Xu⁷, Ikhlas A Khan⁸, Shilin Yang⁹

Affiliations

¹ College of Pharmaceutical Science, Bozhou Vocational and Technical College, Bozhou, 236800, China; College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
² Department I of Pharmacology, Clinical Pharmacology, Cologne University Hospital, Cologne, 50931, Germany.
³ National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, MS, 38677, USA.
⁴ College of Pharmaceutical Science, Bozhou Vocational and Technical College, Bozhou, 236800, China.
⁵ College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
⁶ College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China. Electronic address: liuyanli@suda.edu.cn.
⁷ College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China. Electronic address: xuqiongming@suda.edu.cn.
⁸ College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China; National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, MS, 38677, USA.
⁹ College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China; College of Pharmaceutical Science, College of Pharmaceutical Science, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330006, China.

PMID: 30125848
DOI: 10.1016/j.phytochem.2018.08.009

Abstract

Six undescribed withanolides were isolated and characterized during the investigation of anti-inflammatory and cytotoxic constituents from the whole plants of Physalis minima L. Their structures were elucidated by extensive spectroscopic analyses (IR, UV, HR-ESI-MS, 1D-NMR, and 2D-NMR), and their anti-inflammatory and cytotoxic activities were evaluated in vitro. All the compounds exhibited anti-inflammatory ability via inhibiting the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Moderate cytotoxic activities against A549 lung adenocarcinoma cells, SMMC-7721 hepatic carcinoma cells and MCF-7 breast cancer cells with IC₅₀ values in the range of 40.01-82.17 μM were observed for these withanolides.

Keywords: Anti-inflammatory; Cytotoxic activity; Physalis minima L. (Solanaceae); Withanolides.

MeSH terms

Animals
Anti-Inflammatory Agents / chemistry
Anti-Inflammatory Agents / isolation & purification
Anti-Inflammatory Agents / pharmacology*
Antineoplastic Agents, Phytogenic / chemistry
Antineoplastic Agents, Phytogenic / isolation & purification
Antineoplastic Agents, Phytogenic / pharmacology*
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Humans
Lipopolysaccharides / antagonists & inhibitors
Lipopolysaccharides / pharmacology
Mice
Molecular Conformation
Nitric Oxide / biosynthesis*
Physalis / chemistry*
Plant Extracts / chemistry
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
RAW 264.7 Cells
Structure-Activity Relationship
Withanolides / chemistry
Withanolides / isolation & purification
Withanolides / pharmacology*

Substances

Anti-Inflammatory Agents
Antineoplastic Agents, Phytogenic
Lipopolysaccharides
Plant Extracts
Withanolides
Nitric Oxide