Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway

Lijun Yang; Kaiyan Sun; Jie Chu; Yunhui Qu; Xue Zhao; Huiqing Yin; Liang Ming; Junhu Wan; Fucheng He

doi:10.1016/j.biopha.2018.07.129

Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway

Biomed Pharmacother. 2018 Oct:106:1570-1577. doi: 10.1016/j.biopha.2018.07.129. Epub 2018 Jul 27.

Authors

Lijun Yang¹, Kaiyan Sun², Jie Chu³, Yunhui Qu⁴, Xue Zhao⁵, Huiqing Yin⁶, Liang Ming⁷, Junhu Wan⁸, Fucheng He⁹

Affiliations

¹ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: 1907529740@qq.com.
² Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: 1348580389@qq.com.
³ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: 504017950@qq.com.
⁴ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: zdqyh@126.com.
⁵ Institute of Medical and Pharmaceutical Science, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: 603126271@qq.com.
⁶ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: yihuiqing12@126.com.
⁷ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: mingliang@zzu.edu.cn.
⁸ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: wanjh2009@163.com.
⁹ Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address: hefucheng@zzu.edu.cn.

PMID: 30119232
DOI: 10.1016/j.biopha.2018.07.129

Abstract

AIMS：: Recent research showed that Long non-protein coding RNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) plays a crucial role in the course of tumor formation. The present study was aimed to explore its role in esophageal squamous cell carcinoma (ESCC).

Main methods: Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to examine the expression levels of FTH1P3, mRNA SP1 and NF-kB in ESCC samples and cell lines. The impact of FTH1P3 knockdown was evaluated by WST-1 assays, colony formation assays, scratch wound assays, migration and invasion assays.

Key findings: FTH1P3 was significantly upregulated in ESCC tissues and cells (P < 0.001). Knockdown of FTH1P3 notably decreased the proliferation, migration, and invasion capacity of ESCC cells. Silencing of FTH1P3 decreased the expression of specificity protein 1 (Sp1) and NF-kB (p65) in EC9706 and EC1.

Significance: FTH1P3 plays a crucial role in ESCC tumorigenesis, and can be used as a potential therapeutic target for ESCC.

Keywords: Biological function; Esophageal squamous cell carcinoma; FTH1P3; Long non-coding RNA.

MeSH terms

Aged
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism*
Carcinoma, Squamous Cell / secondary
Cell Line, Tumor
Cell Movement*
Cell Proliferation
Esophageal Neoplasms / genetics
Esophageal Neoplasms / metabolism*
Esophageal Neoplasms / pathology
Esophageal Squamous Cell Carcinoma
Female
Gene Expression Regulation, Neoplastic
Humans
Lymphatic Metastasis
Male
Middle Aged
NF-kappa B / metabolism*
Neoplasm Invasiveness
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
Signal Transduction
Sp1 Transcription Factor / genetics
Sp1 Transcription Factor / metabolism*
Time Factors
Up-Regulation

Substances

NF-kappa B
RNA, Long Noncoding
Sp1 Transcription Factor
SP1 protein, human
long non coding RNA FTH1P3, human