Enhanced photorespiration in transgenic rice over-expressing maize C4 phosphoenolpyruvate carboxylase gene contributes to alleviating low nitrogen stress

Abstract

The objective of this study was to reveal the physiological and molecular mechanisms of low-nitrogen (N) tolerance in transgenic plant lines containing C₄ phosphoenolpyruvate carboxylase (C₄-PEPC) gene. The transgenic rice lines only over-expressing the maize C₄-PEPC) (PC) and their untransformed wild type, Kitaake (WT), were used in this study. At different N levels, the dry weight, total N content, carbon and N levels, photorespiration-related enzymatic activities, gene expression levels and photorespiration-related product accumulations were measured, as were the transgenic lines' agronomic traits. The PC line, having lower total N and higher soluble sugar contents, was more tolerant to low-N stress than WT, which was consistent with its higher PEPC and lower N-assimilation-related enzyme activity levels. The photosynthetic parameters, enzymatic activity levels, transcripts and products related to photorespiration in PC were also greater than in WT under low-N conditions. This study showed that increased carbon levels in transgenic rice lines overexpressing C₄-PEPC could help regulate the photorespiratory pathway under low-N conditions, conferring low-N tolerance and a higher grain yield per plant.

Keywords: Low-nitrogen stress; Phosphoenolpyruvate carboxylase; Photorespiration; Transgenic C(4)-PEPC-Overexpressing rice (Oryza sativa L.).