Development of a TaqMan-based real-time PCR for detecting duck adenovirus 3

J Virol Methods. 2018 Nov:261:86-90. doi: 10.1016/j.jviromet.2018.08.011. Epub 2018 Aug 13.

Abstract

Recently, a novel duck adenovirus (designated as duck adenovirus 3, DAdV-3) was discovered in Muscovy ducks, China. Here, we developed a TaqMan-based real-time PCR assay (qPCR) for the detection of DAdV-3 infection. After the optimization of the qPCR conditions, the lower limit of detection for DAdV-3 infection was 40.9 copies/μl. No cross-reactivity was observed with other duck-derived pathogens. Intra- and inter-assay variability was less than 2.30%. DAdV-3 was detected in embryos and newly hatched ducklings by qPCR assay, the findings provided evidence of possible vertical transmission of DAdV-3. The developed qPCR analysis showed high specificity, sensitivity, and reproducibility, thereby indicating that it can be used in future investigations on the pathogenesis and epidemiology of DAdV-3.

Keywords: DAdV-3; Detection; Real-time PCR assay; TaqMan-based.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae Infections / diagnosis
  • Adenoviridae Infections / veterinary*
  • Adenoviridae Infections / virology
  • Animals
  • Aviadenovirus / genetics
  • Aviadenovirus / isolation & purification*
  • Bird Diseases / diagnosis*
  • Bird Diseases / virology*
  • China
  • Ducks*
  • Infectious Disease Transmission, Vertical
  • Molecular Diagnostic Techniques / methods*
  • Real-Time Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity