Objective: To investigate the effects and its potential mechanism of IKK2 inhibitor LY2409881 on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.
Methods: CCK8 assay was used to detect the effect of LY2409881 on proliferation of DLBCL cell lines; Flow cytometry was used to analyze cell cycle; Western blot was used to analyze apoptosis and its potential mechanism.
Results: LY2409881 inhibited the proliferation of multiple DLBCL cell lines obviously, and caused G1 cell arrest. Furtherly, LY2409881 inhibited the expression of c-FLIP, induced the activation of DR4 and caspase 8. Meanwhile, LY2409881 induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1 and BCL-2.
Conclusion: LY2409881 inhibits the proliferation of DLBCL cell lines, causes G1 cell arrest and induces apoptosis via the endogenous and exogenous apoptotic pathways.