What's in a (Sub)strain?

Jill M Goldstein; Amy J Wagers

doi:10.1016/j.stemcr.2018.07.011

What's in a (Sub)strain?

Stem Cell Reports. 2018 Aug 14;11(2):303-305. doi: 10.1016/j.stemcr.2018.07.011.

Authors

Jill M Goldstein¹, Amy J Wagers²

Affiliations

¹ Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA; Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
² Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA; Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA; Joslin Diabetes Center, Boston, MA 02215, USA. Electronic address: amy_wagers@harvard.edu.

Abstract

C57BL/6J and C57BL/6N inbred mice are widely, and often interchangeably, used for stem cell research; yet, these substrains harbor discrete genetic differences that can cause phenotypic disparities. In this issue of Stem Cell Reports, Morales-Hernández et al. identify one particular difference-disruption of Nicotinamide Nucleotide Transhydrogenase (Nnt)-that increases reactive oxygen exposure and impairs hematopoietic progenitor cell function in C57BL/6J, as compared to C57BL/6N, mice.

Publication types

Letter

MeSH terms

Animals
Genetic Markers
Hematopoietic Stem Cells / metabolism
Mice
Mice, Inbred C57BL / classification*
Mice, Inbred C57BL / genetics*
Mice, Inbred Strains
NADP Transhydrogenases / genetics
Phenotype

Substances

Genetic Markers
NADP Transhydrogenases