Modular Assembly of Synthetic Secondary Chromosomes

Celine Zumkeller; Daniel Schindler; Torsten Waldminghaus

doi:10.1007/978-1-4939-8675-0_5

Modular Assembly of Synthetic Secondary Chromosomes

Methods Mol Biol. 2018:1837:71-94. doi: 10.1007/978-1-4939-8675-0_5.

Authors

Celine Zumkeller¹, Daniel Schindler^{1

2}, Torsten Waldminghaus³

Affiliations

¹ Chromosome Biology Group, LOEWE Center for Synthetic Microbiology-SYNMIKRO, Philipps-Universität Marburg, Marburg, Germany.
² School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, Manchester, UK.
³ Chromosome Biology Group, LOEWE Center for Synthetic Microbiology-SYNMIKRO, Philipps-Universität Marburg, Marburg, Germany. torsten.waldminghaus@synmikro.uni-marburg.de.

PMID: 30109606
DOI: 10.1007/978-1-4939-8675-0_5

Abstract

The development of novel DNA assembly methods in recent years has paved the way for the construction of synthetic replicons to be used for basic research and biotechnological applications. Questions of how chromosomes need to be constructed to maintain the genetic information can now be answered by a learning-by-building approach. Here, we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in Escherichia coli based on the popular Modular Cloning system (MoClo).

Keywords: DNA libraries; Escherichia coli; Genome engineering; Modular cloning; Oligo design; Synthetic chromosomes; synVicII.

MeSH terms

Chromosomes*
Cloning, Molecular* / methods
Escherichia coli / genetics
Gene Order
Genetic Engineering* / methods
Genetic Vectors / genetics
Software
Synthetic Biology* / methods
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