Hydrolysis of ADP-Ribosylation by Macrodomains

Methods Mol Biol. 2018:1813:215-223. doi: 10.1007/978-1-4939-8588-3_14.

Abstract

ADP-ribosylation is the process of transferring the ADP-ribose moiety from NAD+ to a substrate. While a number of proteins represent well described substrates accepting ADP-ribose modification, a recent report demonstrated biological role for DNA ADP-ribosylation as well. The conserved macrodomain fold of several known hydrolyses was found to possess de-ADP-ribosylating activity and the ability to hydrolyze (reverse) ADP-ribosylation. Here we summarize the methods that can be employed to study mono-ADP-ribosylation hydrolysis by macrodomains.

Keywords: ADP-ribosylation; Hydrolysis; Macrodomain fold; NAD+; Toxin-antitoxin system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP Ribose Transferases / chemistry
  • ADP Ribose Transferases / genetics
  • ADP-Ribosylation*
  • Adenosine Diphosphate Ribose / chemistry*
  • Hydrolysis
  • Models, Molecular
  • Molecular Biology / methods*
  • NAD / chemistry
  • NAD / genetics
  • Protein Domains
  • Protein Processing, Post-Translational
  • Proteins / chemistry*
  • Proteins / genetics

Substances

  • Proteins
  • NAD
  • Adenosine Diphosphate Ribose
  • ADP Ribose Transferases