Synthetic Biology Approaches to the Sustainable Production of p-Coumaric Acid and Its Derivatives in Cyanobacteria

Yong Xue; Qingfang He

doi:10.1007/978-981-13-0854-3_11

Synthetic Biology Approaches to the Sustainable Production of p-Coumaric Acid and Its Derivatives in Cyanobacteria

Adv Exp Med Biol. 2018:1080:261-277. doi: 10.1007/978-981-13-0854-3_11.

Authors

Yong Xue¹, Qingfang He²

Affiliations

¹ Division of Microbiology, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, USA.
² Department of Biology, University of Arkansas at Little Rock, Little Rock, AR, USA. qfhe@ualr.edu.

PMID: 30091099
DOI: 10.1007/978-981-13-0854-3_11

Abstract

The photosynthetic cyanobacteria are promising candidates for the sustainable production of a plethora of plant secondary metabolites, which are beneficial to human health but are difficult to produce and purify in other systems. This chapter focuses on genetic engineering of Synechocystis PCC 6803 for production of p-coumaric acid and its derivatives. Cyanobacterial engineering approaches are briefly reviewed. Strategies to increase production yield are discussed, including codon optimization of genes expressing enzymatic proteins and a laccase-coding gene knockout from Synechocystis genome which degrades polyphenols.

Keywords: Caffeic acid; Cyanobacteria; Laccase; P450 proteins; Phenylpropanoid; Secondary metabolites; p-Coumaric acid.

Publication types

Review

MeSH terms

Coumaric Acids
Metabolic Engineering / methods*
Propionates / metabolism*
Synechocystis* / genetics
Synechocystis* / metabolism
Synthetic Biology / methods*

Substances

Coumaric Acids
Propionates
p-coumaric acid