Development of recombinant goatpox virus expressing Echinococcus granulosus EG95 vaccine antigen

Abstract

Goatpox disease and cystic hydatidosis may be simultaneously endemic in a given area. Their pathogens are goatpox virus (GPV) and Echinococcus granulosus (E. granulosus), respectively. Both E. granulosus EG95 subunit vaccine and live-attenuated GPV AV41 vaccine have been widely used for prevention of both diseases in China. However, it has been rarely reported that a bivalent vaccine is developed to prevent both diseases. The GPV is an ideal vector for developing recombinant multivalent vaccines to deliver immunogenic proteins in animals. In this study, we constructed an EG95 antigen-expressing recombinant GPV by the thymidine kinase gene-based homologous recombination in vitro. The recombinant GPV was purified and then proven to be able to express the EG95 antigen in vitro by Western blot and indirect immunoinfluscent assay, therefore expecting its potential as a bivalent vaccine candidate against both diseases in a future animal experiment.

Keywords: EG95 antigen; Homologous recombination; Protein expression; Purification; Recombinant goatpox virus.