Objective: To study the intervention effect of Chloriti Lapis in PTZ-kindled epileptic rat.
Methods: Rats were kindled by pentylenetetrazol( PTZ),and successful kindled model were administered with drugs, then taken out the hippocampus of the brain. HE staining method was used to observe lesion in hippocampus, immunohistochemical method was used to test protein expression of nNOS, xanthine oxidase method was used to measure the activity of T-SOD, thiobarbituric acid method was used to measure the content of MDA,and phosphorus determination method was used to detect the activities of Na+,K+-ATPase and Ca2 +,Mg2 +-ATPase.
Results: Each group of Chloriti Lapis( powder group, dregs group and decoction group) decreased the lesion grade, MDA content,nNOS protein expression, while increased the T-SOD activities, Na+,K+-ATPase and Ca2 +,Mg2 +-ATPase activities in the hippocampu of rats.
Conclusion: Chloriti Lapis have antiepileptic effects, the mechanism may be related to increasing brain antioxidant activities, eliminating free radicals, protecting membrane function, maintaining dynamic balance of ion concentration in the braiofn rat, inhibiting brain abnormalities discharge, and ultimately achieve the goal of epilepsy treatment.