Towards better understanding of industrial cell factories: novel approaches for 13C metabolic flux analysis in complex nutrient environments

Susanne Katharina Schwechheimer; Judith Becker; Christoph Wittmann

doi:10.1016/j.copbio.2018.07.001

Towards better understanding of industrial cell factories: novel approaches for ¹³C metabolic flux analysis in complex nutrient environments

Curr Opin Biotechnol. 2018 Dec:54:128-137. doi: 10.1016/j.copbio.2018.07.001. Epub 2018 Aug 2.

Authors

Susanne Katharina Schwechheimer¹, Judith Becker¹, Christoph Wittmann²

Affiliations

¹ Institute of Systems Biotechnology, Saarland University, Germany.
² Institute of Systems Biotechnology, Saarland University, Germany. Electronic address: christoph.wittmann@uni-saarland.de.

PMID: 30077860
DOI: 10.1016/j.copbio.2018.07.001

Abstract

The quantification of intracellular fluxes via ¹³C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, ¹³C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medium compounds. The use of parallel ¹³C tracer experiments as well as analytical multi-readout of labeling data together with model-based simulation offer a great starting point to tackle such complex systems. Prominent examples will highlight the recent progress made.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Antibody Formation
Biotechnology / methods*
Carbon Isotopes / metabolism*
Isotope Labeling / methods
Metabolic Engineering / methods
Metabolic Flux Analysis / methods*

Substances

Carbon Isotopes