CYP1A1 activity in rainbow trout is inhibited by the environmental pollutant p-cresol

Viktoriia Burkina; Sidika Sakalli; Vladimir Zlabek; Galia Zamaratskaia

doi:10.1016/j.etap.2018.07.013

CYP1A1 activity in rainbow trout is inhibited by the environmental pollutant p-cresol

Environ Toxicol Pharmacol. 2018 Sep:62:199-202. doi: 10.1016/j.etap.2018.07.013. Epub 2018 Jul 26.

Authors

Viktoriia Burkina¹, Sidika Sakalli², Vladimir Zlabek², Galia Zamaratskaia³

Affiliations

¹ University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25, Vodnany, Czech Republic; Swedish University of Agricultural Sciences, Department of Molecular Science, P.O. Box 7015, SE-750 07, Uppsala, Sweden. Electronic address: vburkina@frov.jcu.cz.
² University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25, Vodnany, Czech Republic.
³ University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25, Vodnany, Czech Republic; Swedish University of Agricultural Sciences, Department of Molecular Science, P.O. Box 7015, SE-750 07, Uppsala, Sweden.

PMID: 30077163
DOI: 10.1016/j.etap.2018.07.013

Abstract

Naturally- and anthropogenically-produced cresols could pose serious risks to fish health. In this study, three piscine CYP isoforms were investigated for their abilities to interact with p-cresol. Therefore, the activity of 7-ethoxyresorufin-O-deethylase (EROD), 7-benzyloxy-4-trifluoromethylcoumarin O-debenzylase (BFCOD), and p-nitrophenol hydroxylase (PNPH) were evaluated in the hepatic microsomes of juvenile rainbow trout. Results showed that EROD activity was inhibited in a competitive manner, BFCOD activity was inhibited in presence the highest tested p-cresol concentration and PNPH activity was not affected. These results indicate that p-cresol might affect the ability of fish to metabolize numerous aromatic hydrocarbons and dioxin compounds, which are present in the aquatic environment.

Keywords: CYP1A1; CYP2E1-like; CYP3A-like; Fish; p-Cresol.

MeSH terms

Animals
Cresols / toxicity*
Cytochrome P-450 CYP1A1 / antagonists & inhibitors*
Cytochrome P-450 CYP1A1 / metabolism
Fish Proteins / metabolism*
Oncorhynchus mykiss / metabolism*
Water Pollutants, Chemical / toxicity*

Substances

Cresols
Fish Proteins
Water Pollutants, Chemical
4-cresol
Cytochrome P-450 CYP1A1