Characterization of a novel allelic variant in HLA-B*46:01 lineage, HLA-B*46:01:25, by cloning, phasing and sequencing

Int J Immunogenet. 2018 Dec;45(6):347-350. doi: 10.1111/iji.12392. Epub 2018 Jul 31.

Abstract

A new allelic variant in HLA-B*46:01 lineage, HLA-B*46:01:25, has been identified in a male individual of Mongol ethnicity residing in northern China. Following polymerase chain reaction-sequence-based typing (PCR-SBT), this novel variant was further confirmed by cloning, phasing and sequencing. HLA-B*46:01:25 differs from HLA-B*46:01:01 by a single synonymous T substitution at nucleotide position 137 (C - T) in exon 4, corresponding to codon 228 (ACC-ACT) of the mature HLA-B mRNA molecule.

Keywords: HLA-B*46:01:25; Chinese Mongolian population; cloning; long-range PCR; sequence-based typing.

MeSH terms

  • Alleles*
  • Cloning, Molecular*
  • Codon*
  • Female
  • HLA-B Antigens / genetics*
  • Humans
  • Male
  • Mongolia
  • Point Mutation*
  • Sequence Analysis, DNA*

Substances

  • Codon
  • HLA-B Antigens
  • HLA-B*46:01 antigen