Label-free distinction between p53+/+ and p53 -/- colon cancer cells using a graphene based SERS platform

Owen Liang; Pu Wang; Ming Xia; Catherine Augello; Fan Yang; Gang Niu; Huinan Liu; Ya-Hong Xie

doi:10.1016/j.bios.2018.07.038

Label-free distinction between p53+/+ and p53 -/- colon cancer cells using a graphene based SERS platform

Biosens Bioelectron. 2018 Oct 30:118:108-114. doi: 10.1016/j.bios.2018.07.038. Epub 2018 Jul 18.

Authors

Owen Liang¹, Pu Wang², Ming Xia², Catherine Augello³, Fan Yang², Gang Niu⁴, Huinan Liu⁵, Ya-Hong Xie⁶

Affiliations

¹ Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, United States; Electronic Materials Research Laboratory, Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
² Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, United States.
³ Department of Bioengineering, University of California, Riverside, Riverside, CA 92521, United States.
⁴ Electronic Materials Research Laboratory, Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
⁵ Department of Bioengineering, University of California, Riverside, Riverside, CA 92521, United States; Cell, Molecular and Developmental Biology (CMDB) Program, and Biomedical Sciences Program of School of Medicine, University of California, Riverside, Riverside, CA 92507, United States.
⁶ Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, United States; Electronic Materials Research Laboratory, Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, China; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA 90024, United States. Electronic address: yhx@ucla.edu.

PMID: 30059864
DOI: 10.1016/j.bios.2018.07.038

Abstract

Surface-Enhanced Raman Scattering (SERS) is used to differentiate two colon cancer cell line HCT 116, that is, to distinguish a TP53 gene knockout cell line (p53 -/-) from a wild type (p53 +/+). A label-free graphene/gold nanopyramid based SERS platform, combined with the multivariate analysis: principal component analysis, is used to profile live, dead, and burst colon cancer cells suspended in simulated body fluid (SBF). The graphene sheet permits SERS hotspot identification and provides a chemical enhancement for the biological constituents. This study found that a unique fingerprint exists for three different states of the cell, burst, live, and dead, which were used to differentiate the p53 +/+ and p53 -/- cell lines. Perceptron with Pocket Algorithm was also coupled with PCA to demonstrate an average of 81% sensitivity and 97% specificity in separating the two cell lines. The demonstration of single gene differentiation shows the great applicable potential of this SERS graphene hybrid platform for cancer diagnosis.

Keywords: Cancer; Colon; Graphene; Label-Free; SERS; p53.

MeSH terms

Biosensing Techniques / instrumentation*
Biosensing Techniques / methods*
Colonic Neoplasms / diagnosis*
Graphite / chemistry*
Humans
Metal Nanoparticles
Spectrum Analysis, Raman*
Tumor Suppressor Protein p53 / genetics*

Substances

Tumor Suppressor Protein p53
Graphite