Tetrahedral DNAzymes for enhanced intracellular gene-silencing activity

Chem Commun (Camb). 2018 Aug 21;54(68):9410-9413. doi: 10.1039/c8cc05721d.

Abstract

We prepared tetrahedral DNAzymes (TDzs) to overcome potential limitations such as insufficient serum stability and poor cellular uptake of single-stranded DNAzymes (ssDzs). TDzs showed enhanced serum stability and higher cellular uptake efficiency compared to those of ssDzs, providing significantly improved intracellular gene-silencing activity to down-regulate the target mRNA level.

MeSH terms

  • Animals
  • Cattle
  • DNA, Catalytic / chemistry
  • DNA, Catalytic / genetics*
  • DNA, Single-Stranded / chemistry
  • DNA, Single-Stranded / genetics
  • Down-Regulation
  • Fibroblasts / metabolism
  • Gene Silencing*
  • Kinetics
  • Mice
  • NIH 3T3 Cells
  • Nanostructures / chemistry
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • DNA, Catalytic
  • DNA, Single-Stranded
  • RNA, Messenger