Abstract
We prepared tetrahedral DNAzymes (TDzs) to overcome potential limitations such as insufficient serum stability and poor cellular uptake of single-stranded DNAzymes (ssDzs). TDzs showed enhanced serum stability and higher cellular uptake efficiency compared to those of ssDzs, providing significantly improved intracellular gene-silencing activity to down-regulate the target mRNA level.
MeSH terms
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Animals
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Cattle
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DNA, Catalytic / chemistry
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DNA, Catalytic / genetics*
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DNA, Single-Stranded / chemistry
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DNA, Single-Stranded / genetics
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Down-Regulation
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Fibroblasts / metabolism
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Gene Silencing*
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Kinetics
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Mice
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NIH 3T3 Cells
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Nanostructures / chemistry
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Nucleic Acid Hybridization
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
Substances
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DNA, Catalytic
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DNA, Single-Stranded
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RNA, Messenger