Fluorescent chemodosimeter for quantification of cystathionine-γ-synthase activity in plant extracts and imaging of endogenous biothiols

Chem Commun (Camb). 2018 Aug 9;54(65):9079-9082. doi: 10.1039/c8cc04296a.

Abstract

A new reagent for quantification of CgS in plant extracts using a generalized methodology suitable for recognition of homocysteine (Hcy) with luminescence ON response.

MeSH terms

  • Animals
  • Benzoxazines / chemical synthesis
  • Benzoxazines / chemistry
  • Benzoxazines / pharmacology*
  • Carbon-Oxygen Lyases / analysis*
  • Cysteine / analysis
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / pharmacology*
  • HeLa Cells
  • Homocysteine / analysis
  • Humans
  • Kinetics
  • Limit of Detection
  • Microscopy, Confocal / methods
  • Microscopy, Fluorescence / methods
  • Nicotiana / chemistry
  • Plant Extracts / analysis*
  • Zebrafish

Substances

  • Benzoxazines
  • Fluorescent Dyes
  • Plant Extracts
  • Homocysteine
  • O-succinylhomoserine (thiol)-lyase
  • Carbon-Oxygen Lyases
  • Cysteine