Biological nitrogen removal and metabolic characteristics of a novel aerobic denitrifying fungus Hanseniaspora uvarum strain KPL108

Haihan Zhang; Zhenfang Zhao; Pengliang Kang; Yue Wang; Ji Feng; Jingyu Jia; Zhonghui Zhang

doi:10.1016/j.biortech.2018.07.073

Biological nitrogen removal and metabolic characteristics of a novel aerobic denitrifying fungus Hanseniaspora uvarum strain KPL108

Bioresour Technol. 2018 Nov:267:569-577. doi: 10.1016/j.biortech.2018.07.073. Epub 2018 Jul 25.

Authors

Haihan Zhang¹, Zhenfang Zhao², Pengliang Kang², Yue Wang², Ji Feng², Jingyu Jia², Zhonghui Zhang²

Affiliations

¹ Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, People's Republic of China; Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, People's Republic of China; School of Environmental and Municipal Engineering, Institute of Environmental Microbial Technology, Xi'an University of Architecture and Technology, Xi'an 710055, People's Republic of China. Electronic address: zhanghaihan@xauat.edu.cn.
² Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, People's Republic of China; Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, People's Republic of China; School of Environmental and Municipal Engineering, Institute of Environmental Microbial Technology, Xi'an University of Architecture and Technology, Xi'an 710055, People's Republic of China.

PMID: 30053715
DOI: 10.1016/j.biortech.2018.07.073

Abstract

A novel aerobic denitrifying fungal strain KPL108 was isolated from the sediment of Jinpen drinking water reservoir and identified as Hanseniaspora uvarum. Strain KPL108 removed 99% of nitrate without nitrite accumulation under aerobic conditions, while the total organic carbon removal efficiency was 93%. KPL108 expressed fungal specific denitrifying gene p450nor. Nitrogen balance exhibited that approximately 92% of the initial nitrate was removed as gaseous products. Based on ¹³C-isotope labeling tracer, pentose phosphate pathway and tricarboxylic acid cycle were highly active in intracellular central carbon metabolism of strain KPL108. Response surface methodology revealed that the maximum total nitrogen removal efficiency occurred with the optimized parameters: C/N ratio of 6.4, pH of 8.2, 28.5 °C and 109.7 rpm. Collectively, the results from the present study indicate that strain KPL108 has aerobic denitrification ability, which has a great potential application for nitrogenous wastewater treatment.

Keywords: Aerobic denitrifying fungus; Hanseniaspora uvarum; Metabolic characteristics; Nitrogen removal; Response surface methodology.

MeSH terms

Aerobiosis
Denitrification*
Hanseniaspora*
Nitrates
Nitrites
Nitrogen

Substances

Nitrates
Nitrites
Nitrogen