Hypericin can cross barriers in the chicken's chorioallantoic membrane model when delivered in low-density lipoproteins

Abstract

Background: Low-density lipoproteins (LDL) were used as a natural drug delivery system for the transport of hypericin (Hyp) in the bloodstream of the chicken's chorioallantoic membrane model (CAM). Hyp was chosen as a model for hydrophobic drug used in photo-diagnosis and photo-treatments (PDT). The extravasation of the Hyp:LDL complexes for different concentration ratios and the redistribution of Hyp between different serum components were investigated with an innovative statistical treatment.

Methods: Hyp biodistribution was monitored in CAM by intravital fluorescence microscopy. The innovative statistical treatment of experimental data presented here enabled us to obtain highly detailed information from the weak Hyp fluorescence distribution in CAM blood vessels. Hyp redistribution between the serum components was studied by fluorescence spectroscopy in lipids/protein composed solutions.

Results: Extravasation of Hyp was dependent on Hyp:LDL concentration ratio. While Hyp:LDL = 50:1 resulted in a significant Hyp extravasation, the Hyp extravasation from Hyp:LDL = 100:1 was weak. The redistribution of Hyp was found to be faster for lipidic particles than for proteins.

Conclusion: We have demonstrated that lipids composition has a significant control over Hyp delivery in CAM.

Keywords: Biodistribution; Bootstrapping and bagging; Hypericin; In ovo; Lipoproteins.