A lipid droplet-targeted fluorescence probe for visualizing exogenous copper (II) based on LLCT and LMCT

Ying Xia; Tingting Yu; Fei Li; Weiju Zhu; Yinggang Ji; Shuai Kong; Chunxia Li; Bei Huang; Xuanjun Zhang; Yupeng Tian; Hongping Zhou

doi:10.1016/j.talanta.2018.05.080

A lipid droplet-targeted fluorescence probe for visualizing exogenous copper (II) based on LLCT and LMCT

Talanta. 2018 Oct 1:188:178-182. doi: 10.1016/j.talanta.2018.05.080. Epub 2018 May 26.

Authors

Ying Xia¹, Tingting Yu¹, Fei Li¹, Weiju Zhu¹, Yinggang Ji¹, Shuai Kong¹, Chunxia Li², Bei Huang², Xuanjun Zhang³, Yupeng Tian¹, Hongping Zhou⁴

Affiliations

¹ College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, AnHui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, 230601 Hefei, PR China.
² Center of Stem cell Research and Transformation Medicine, Anhui University, Hefei 230601, PR China.
³ Faculty of Health Sciences, University of Macau, Taipa, Macau SAR, PR China.
⁴ College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, AnHui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, 230601 Hefei, PR China. Electronic address: zhpzhp@263.net.

PMID: 30029361
DOI: 10.1016/j.talanta.2018.05.080

Abstract

A simple and sensitive probe, pyridine-based Schiff base (PBSB), is prepared based on the previous studies, which shows a rapid response to copper (II) through a fluorescence enhancement process. On association with copper (II), PBSB immediately presents a large red-shift (130 nm) in emission spectrum simultaneously accompanying with increasing emission intensity. The mechanism and binding sites of PBSB detecting copper (II) have been established by theoretical calculations and ¹HNMR study, respectively. More importantly, in the complex biological system, PBSB having an excellent membrane permeability and a great photostability can visualize copper (II) in lipid droplet by bio-imaging, which display so great promising advantages in the issues causing by disorder of copper ions that could be convenient for prevention and control problems with human health and complex bio-system.

Keywords: Biological application; Fluorescence probe; LLCT and LMCT; Lipid droplet; Recognition mechanism.