Effect of inoculation with arbuscular mycorrhizal fungi and blanching on the bioaccessibility of heavy metals in water spinach (Ipomoea aquatica Forsk.)

Abstract

A plant's tolerance to heavy metals (HMs) and its detoxification mechanisms are associated with the subcellular distribution of HMs and their chemical forms. In this study, water spinach (Ipomoea aquatica Forsk.) was grown in two soils contaminated with a single HM (cadmium, Cd) or combined HMs (Cd and nickel, Ni). Inoculation of arbuscular mycorrizal fungi (AMF) was conducted to increase the accumulation of phosphorus (P) in plants. One major exception was to decrease the migration and accumulation of HMs in edible parts by the formation of P-HM complexes. The effects of blanching and simulated digestion on bioaccessibility were also assessed. The experimental results showed that the water spinach species used in this study had a high capacity to accumulate HMs. AMF treatment improved water spinach growth and decreased the accumulation of Ni but not that of Cd. Soluble and inorganic Cd and Ni were the major subcellular fractions and chemical forms in water spinach; these two HMs also exhibited higher migration capacities in comparison to chromium (Cr). Relative to raw tissues, 45-84% of Cd, Cr, and Ni were leached after blanching. Approximately 32-55%, 16-50%, and 27-40% of Cd, Cr, and Ni, respectively, were bioaccessible and could be metabolized by in vitro digestive fluids.

Keywords: Bioaccessibility; Chemical form; Heavy metal; Risk assessment; Subcellular distribution.