Sequence data of six unusual alleles at SE33 and D1S1656 STR Loci

Hussain M Alsafiah; Arati Iyengar; Sibte Hadi; Waleed M Alshlash; William Goodwin

doi:10.1002/elps.201800191

Sequence data of six unusual alleles at SE33 and D1S1656 STR Loci

Electrophoresis. 2018 Oct;39(19):2471-2476. doi: 10.1002/elps.201800191. Epub 2018 Aug 12.

Authors

Hussain M Alsafiah^{1

2}, Arati Iyengar¹, Sibte Hadi¹, Waleed M Alshlash², William Goodwin¹

Affiliations

¹ University of Central Lancashire-School of Forensic and Applied Sciences, Preston, Lancashire, United Kingdom of Great Britain and Northern Ireland.
² Forensic Genetics Laboratory, General Administration of Criminal Evidences, Public Security, Ministry of Interior, Kingdom of Saudi Arabia.

PMID: 30028539
DOI: 10.1002/elps.201800191

Abstract

When profiling a reference dataset of 500 DNA samples for the population of Saudi Arabia, using the GlobalFiler® PCR amplification kit, six unusual alleles were detected. At the SE33 locus, four novel alleles were found: 2, 14.3, 20.3, and 38; two alleles at the D1S1656 locus: 7 and 8 had been previously reported, but no published sequence data was available. The D1S1656 alleles were sequenced using ForenSeq™ DNA Signature Prep with the MiSeq FGx System (Illumina, USA). As the SE33 is not reported by available Massively Parallel Sequencing (MPS) systems, samples that exhibited the unreported alleles were sequenced using BigDye™ Terminator v3.1 Cycle Sequencing Kit. Here we present the sequence and structure of the previously uncharacterized alleles.

Keywords: D1S1656; Massively Parallel Sequencing; SE33; STR variant alleles; Saudi Arabian population.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Databases, Genetic
Genetic Loci / genetics*
High-Throughput Nucleotide Sequencing / methods*
Humans
Microsatellite Repeats / genetics*
Polymerase Chain Reaction
Racial Groups / genetics
Saudi Arabia
Sequence Analysis, DNA / methods*