A stable zinc(II)-mediated base pair in a parallel-stranded DNA duplex

Biswarup Jash; Jens Müller

doi:10.1016/j.jinorgbio.2018.07.002

A stable zinc(II)-mediated base pair in a parallel-stranded DNA duplex

J Inorg Biochem. 2018 Sep:186:301-306. doi: 10.1016/j.jinorgbio.2018.07.002. Epub 2018 Jul 5.

Authors

Biswarup Jash¹, Jens Müller²

Affiliations

¹ Westfälische Wilhelms-Universität Münster, Institut für Anorganische und Analytische Chemie, NRW Graduate School of Chemistry, Corrensstraße 28/30, 48149 Münster, Germany.
² Westfälische Wilhelms-Universität Münster, Institut für Anorganische und Analytische Chemie, NRW Graduate School of Chemistry, Corrensstraße 28/30, 48149 Münster, Germany. Electronic address: mueller.j@uni-muenster.de.

PMID: 30005358
DOI: 10.1016/j.jinorgbio.2018.07.002

Abstract

A stable zinc(II)-mediated base pair was formed within a parallel-stranded DNA duplex comprising a GNA (glycol nucleic acid) functionalized nucleoside analog containing the artificial nucleobase 1H-imidazo[4,5-f][1,10]phenanthroline (P). The formation of the metal-mediated base pair was confirmed by UV and CD spectroscopic analyses. In the Zn(II)-mediated homo base pairs of the type P-Zn(II)-P, the metal ion adopts a [2 + 2] coordination environment. CD spectroscopic data suggest that the chiral metal complex is formed enantiospecifically, likely induced by the helical chirality of the surrounding DNA duplex. The Zn(II)-mediated base pair stabilizes the DNA oligonucleotide duplex by 9 °C. This stable Zn(II)-mediated base pair within a parallel-stranded duplex extends the scope of site-specific functionalization of nucleic acids by virtue of metal-mediated base pairing.

Keywords: DNA; Metal-mediated base pair; Phenanthroline; Zinc.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pairing*
DNA / chemistry*
Models, Molecular*
Nucleosides / chemistry*
Zinc / chemistry*

Substances

Nucleosides
DNA
Zinc