The article confirms the possibility of simultaneous implementation of custom-made mitral and tricuspid valve prostheses together with their subvalvular apparatuses from extracellular matrix. Both prostheses were implanted in a 43-year-old man experiencing severe mitral and tricuspid regurgitation, decreased left ventricle ejection fraction, acromegaly, macrocardia and a history of undiagnosed infective process. Postoperative echocardiography showed good haemodynamic function of implanted valves. On postoperative day 11, a paravalvular leak of mitral valve was noticed and required reoperation. The predischarge echocardiography showed no paravalvular leak. The patient was discharged in good condition 52 days after the surgery. The custom-made extracellular matrix valves do not require postoperative anticoagulation. They also serve as prostheses of subvalvular apparatus and, therefore, ameliorate the functioning of ventricles. They prevent the postoperative adverse ventricular remodelling as well. Despite good early haemodynamic results, extracellular matrix mitral valve prosthesis requires further study to assess its long-term function.