Abstract
Long noncoding RNAs (lncRNAs) have been emerging as master regulators of tumor growth and metastasis, but the functions and underlying mechanisms of lncRNAs in colorectal cancer (CRC) still need to be clarified. Here, we found a novel lncRNA u50535, which was greatly overexpressed in CRC tissues and was associated with poor prognosis in CRC patients. Function studies showed that u50535 was an oncogene in CRC both in vitro and in vivo. In mechanism, through RNA sequencing and rescue assay, we found that u50535 activates CCL20 signaling to promote cell proliferation and migration in CRC. Taken together, these findings suggest that u50535 can promote CRC growth and metastasis and may serve as a potential biomarker in CRC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / genetics
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Apoptosis / physiology
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Cell Cycle / genetics
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Cell Cycle / physiology
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Cell Movement / genetics
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Cell Movement / physiology
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Cell Proliferation / genetics
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Cell Proliferation / physiology
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Chemokine CCL20 / metabolism*
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Colorectal Neoplasms / genetics
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Colorectal Neoplasms / metabolism*
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Colorectal Neoplasms / pathology*
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Epithelial-Mesenchymal Transition / genetics
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Epithelial-Mesenchymal Transition / physiology
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Female
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Gene Expression Regulation, Neoplastic / genetics
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Gene Expression Regulation, Neoplastic / physiology
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HCT116 Cells
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Humans
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In Situ Hybridization, Fluorescence
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Middle Aged
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RNA, Long Noncoding / genetics
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RNA, Long Noncoding / metabolism*
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Signal Transduction / genetics
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Signal Transduction / physiology
Substances
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Chemokine CCL20
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RNA, Long Noncoding