A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures

D Septiadi; J Bourquin; E Durantie; A Petri-Fink; B Rothen-Rutishauser

doi:10.1038/s41598-018-28206-2

A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures

Sci Rep. 2018 Jun 29;8(1):9861. doi: 10.1038/s41598-018-28206-2.

Authors

D Septiadi¹, J Bourquin², E Durantie², A Petri-Fink^{2

3}, B Rothen-Rutishauser⁴

Affiliations

¹ Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700, Fribourg, Switzerland. dedy.septiadi@unifr.ch.
² Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700, Fribourg, Switzerland.
³ Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700, Fribourg, Switzerland.
⁴ Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700, Fribourg, Switzerland. barbara.rothen@unifr.ch.

Abstract

Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alveolar Epithelial Cells / cytology
Alveolar Epithelial Cells / physiology*
Cell Adhesion
Cell Culture Techniques / methods*
Cell Movement
Cells, Cultured
Coculture Techniques
Dendritic Cells / cytology
Dendritic Cells / physiology*
Humans
Image Processing, Computer-Assisted
Imaging, Three-Dimensional / methods*
Macrophages / cytology
Macrophages / physiology*
Microscopy, Fluorescence
Nanoparticles / chemistry
Time-Lapse Imaging / methods*
Wound Healing