Anti-biofilm Activity Against Gram-Positive Bacteria by Biologically Synthesized Silver Nanoparticles Using Curcuma longa

Abstract

Objective: To assess the biofilm inhibition activity of biosynthesized silver nanoparticles (AgNPs) using Curcuma longa.

Method: Characterizations of these biogenic nanoparticles were done by UV-Vis spectroscopy, TEM, EDX and nanoparticles tracking analyzer. The antibiofilm activity of AgNPs against S. aureus and S. pneumoniae was carried out by 96 well plate method. Further, the combined effect of AgNPs with the antibiotic was studied against S. aureus and S. pneumoniae.

Results: UV-spectroscopic analysis showed a surface plasmon resonance peak at 415nm. The shape of AgNPs is spherical with an average size of 20-40 nm while the elemental silver was confirmed by EDX analysis. These biosynthesized AgNPs have anti-biofilm activity against S. aureus and S. pneumoniae. Furthermore, the combined effect of AgNPs with an antibiotic, showed enhanced anti-biofilm activity against both the strains under study.

Conclusion: C. longa mediated AgNPs showed potent anti-biofilm activity.

Keywords: Anti-biofilm activity; Curcuma longa; UVspectroscopic analysis; characterization; gram-positive bacteria; silver nanoparticles..