Label-free fluorescent aptasensor berberine-based strategy for ultrasensitive detection of Hg2+ ion

Abstract

A label-free fluorescent aptasensing platform was fabricated and a simple and rapid method to detect Hg²⁺ ion in aqueous solution was put forward by means of berberine and Hg²⁺ ion-aptamer are as the fluorescence probe and the recognition element, respectively. Various factors including the concentration of berberine, Hg²⁺ ion and Hg²⁺ ion-aptamer, pH effect and the reaction time were investigated in detail. Under the optimal experimental conditions, in the sensing system, the fluorescence intensity changes displayed a calibration response for Hg²⁺ ion in the range of 0.1 μM to 10.0 μM and the detection limit was of 7.7 nM (S/N = 3). The fabricated label-free fluorescence aptasensor is not only conveniently but also effectively applicable used for analysis of Hg²⁺ ion in blood serum and tap water samples and the recovery range is of 96.0%-105.7%. In brief, this study offers an easy, economical and stable assay system for detecting Hg²⁺ ion in rough condition.

Keywords: Aptasensor; Berberine; Fluorescence; Hg(2+) ion; Label-free.