The effect of the frictional heat on retention and efficiency in thermostated or insulated chromatographic columns packed with sub-2-μm particles

Nándor Lambert; Attila Felinger

doi:10.1016/j.chroma.2018.06.038

The effect of the frictional heat on retention and efficiency in thermostated or insulated chromatographic columns packed with sub-2-μm particles

J Chromatogr A. 2018 Aug 31:1565:89-95. doi: 10.1016/j.chroma.2018.06.038. Epub 2018 Jun 19.

Authors

Nándor Lambert¹, Attila Felinger²

Affiliations

¹ MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary.
² MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary; Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúság útja 6, H-7624 Pécs, Hungary; Institute of Bioanalysis, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, Hungary. Electronic address: felinger@ttk.pte.hu.

PMID: 29941197
DOI: 10.1016/j.chroma.2018.06.038

Abstract

The mass-transfer properties of a core-shell packing material with 1.6 μm particle diameter, and that of a fully porous packing material with 1.7 μm particle diameter were investigated and compared. The first absolute and the second central moments of the peaks of the homologous series of alkylbenzenes, over a wide range of mobile phase velocities were measured and used for the calculation of the mass-transfer coefficients. For the evaluation of the band broadening caused by the thermal dissimilarities, the measurements were carried out under thermostated conditions and also at near adiabatic insulation of the columns.

Keywords: Column efficiency; Frictional heat; Insulation; Sub-2-μm particles; Thermostating; UHPLC.

MeSH terms

Benzene / analysis
Chromatography, High Pressure Liquid / methods*
Friction*
Hot Temperature*
Molecular Weight
Particle Size*
Porosity

Substances

Benzene