Intein-mediated backbone cyclization of entolimod confers enhanced radioprotective activity in mouse models

Bingyu Ye; Wenlong Shen; Minglei Shi; Yan Zhang; Cunshuan Xu; Zhihu Zhao

doi:10.7717/peerj.5043

Intein-mediated backbone cyclization of entolimod confers enhanced radioprotective activity in mouse models

PeerJ. 2018 Jun 18:6:e5043. doi: 10.7717/peerj.5043. eCollection 2018.

Authors

Bingyu Ye^{1

2

3}, Wenlong Shen³, Minglei Shi³, Yan Zhang³, Cunshuan Xu^{1

2}, Zhihu Zhao³

Affiliations

¹ College of Life Science, Henan Normal University, Xinxiang, China.
² State Key Laboratory Cultivation Base for Cell Differentiation Regulation, College of Life Science, Henan Normal University, Xinxiang, China.
³ Beijing Institute of Biotechnology, Beijing, China.

Abstract

Background: Entolimod is a Salmonella enterica flagellin derivate. Previous work has demonstrated that entolimod effectively protects mice and non-human primates from ionizing radiation. However, it caused a "flu-like" syndrome after radioprotective and anticancer clinical application, indicating some type of immunogenicity and toxicity. Cyclization is commonly used to improve the in vivo stability and activity of peptides and proteins.

Methods: We designed and constructed cyclic entolimod using split Nostoc punctiforme DnaE intein with almost 100% cyclization efficiency. We adopted different strategies to purify the linear and circular entolimod due to their different topologies. Both of linear and circular entolimod were first purified by Ni-chelating affinity chromatography, and then the linear and circular entolimod were purified by size-exclusion and ion-exchange chromatography, respectively.

Results: The circular entolimod showed significantly increased both the in vitro NF-κB signaling and in vivo radioprotective activity in mice.

Conclusion: Our data indicates that circular entolimod might be a good candidate for further clinical investigation.

Keywords: Cyclization; Entolimod; Npu DnaE intein; Radioprotective activity.

Grants and funding

This research was supported by the National Basic Research Program of China (2013CB966802), National Natural Science Foundation of China (31370762, 31030026, 31071119, 81372218) and National Key Technology R&D Program of China (2012BAI01B07). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.