Draft genome sequence of an extended-spectrum β-lactamase (CTX-M-15)-producing Escherichia coli ST10 isolated from a nasal sample of an abattoir worker in Cameroon

Luria Leslie Founou; Raspail Carrel Founou; Mushal Allam; Arshad Ismail; Sabiha Yusuf Essack

doi:10.1016/j.jgar.2018.06.004

Draft genome sequence of an extended-spectrum β-lactamase (CTX-M-15)-producing Escherichia coli ST10 isolated from a nasal sample of an abattoir worker in Cameroon

J Glob Antimicrob Resist. 2018 Sep:14:68-69. doi: 10.1016/j.jgar.2018.06.004. Epub 2018 Jun 19.

Authors

Luria Leslie Founou¹, Raspail Carrel Founou², Mushal Allam³, Arshad Ismail³, Sabiha Yusuf Essack⁴

Affiliations

¹ Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Food Safety and Environmental Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon. Electronic address: luriafounou@gmail.com.
² Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Clinical Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon.
³ Sequencing Core Facility, National Health Laboratory Service, Johannesburg, South Africa.
⁴ Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.

PMID: 29933117
DOI: 10.1016/j.jgar.2018.06.004

Abstract

Objectives: Here we report the draft genome sequence of Escherichia coli strain HN503E1II isolated from a nasal sample of an abattoir worker in Cameroon.

Methods: Bacterial genomic DNA of E. coli HN503E1II was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using the Qiagen CLC Genomics Workbench. The assembled contigs were annotated and antibiotic resistance genes, virulence factors, plasmids and the sequence type (ST) were identified.

Results: The genome comprised a circular chromosome of 4 674201bp, with a 50.78% G+C content and several resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, sulphonamides, trimethoprim, macrolides and tetracycline. The isolate was assigned to ST10 with 100% identity among the seven housekeeping genes. In silico analysis also revealed the presence six plasmid types and one virulence factor.

Conclusion: The genome sequence reported here will provide valuable information for a better understanding of the genetic structure of the E. coli genome in Africa.

Keywords: Antibiotic resistance; ESBL; Escherichia coli; Extended-spectrum β-lactamase; Food chain; Whole-genome sequencing.

Publication types

Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Abattoirs
Anti-Bacterial Agents / pharmacology*
Cameroon
DNA, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics
Escherichia coli / drug effects*
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / microbiology
Female
Genome, Bacterial
Humans
Nose / microbiology*
Plasmids / genetics
Virulence Factors / genetics
Whole Genome Sequencing
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
DNA, Bacterial
Virulence Factors
beta-lactamase CTX-M-15
beta-Lactamases