High-throughput label-free microcontact printing graphene-based biosensor for valley fever

Shih-Ming Tsai; Tyler Goshia; Yen-Chang Chen; Agnes Kagiri; Angelo Sibal; Meng-Hsuen Chiu; Anand Gadre; Vincent Tung; Wei-Chun Chin

doi:10.1016/j.colsurfb.2018.06.011

High-throughput label-free microcontact printing graphene-based biosensor for valley fever

Colloids Surf B Biointerfaces. 2018 Oct 1:170:219-223. doi: 10.1016/j.colsurfb.2018.06.011. Epub 2018 Jun 19.

Authors

Shih-Ming Tsai¹, Tyler Goshia², Yen-Chang Chen³, Agnes Kagiri⁴, Angelo Sibal⁵, Meng-Hsuen Chiu⁶, Anand Gadre⁷, Vincent Tung⁸, Wei-Chun Chin⁹

Affiliations

¹ Bioengineering Program, School of Engineering, University of California-Merced, Merced, 95343, CA, USA. Electronic address: stsai6@ucmerced.edu.
² Bioengineering Program, School of Engineering, University of California-Merced, Merced, 95343, CA, USA. Electronic address: tgoshia@ucmerced.edu.
³ Material Science and Engineering Program, School of Engineering, University of California, Merced, CA, 95343, USA; Organic and Macromolecular Division, Molecular Foundry, Lawrence Berkeley National Lab, Berkeley, CA, 94720, USA. Electronic address: ychen55@ucmerced.edu.
⁴ Bioengineering Program, School of Engineering, University of California-Merced, Merced, 95343, CA, USA. Electronic address: akagiri@ucmerced.edu.
⁵ Bioengineering Program, School of Engineering, University of California-Merced, Merced, 95343, CA, USA. Electronic address: asibal2@ucmerced.edu.
⁶ Bioengineering Program, School of Engineering, University of California-Merced, Merced, 95343, CA, USA. Electronic address: mchiu22@ucmerced.edu.
⁷ Stem Cell Instrumentation Foundry, University of California, Merced, USA. Electronic address: agadre@ucmerced.edu.
⁸ Material Science and Engineering Program, School of Engineering, University of California, Merced, CA, 95343, USA; Organic and Macromolecular Division, Molecular Foundry, Lawrence Berkeley National Lab, Berkeley, CA, 94720, USA. Electronic address: ctung@ucmerced.edu.
⁹ Bioengineering Program, School of Engineering, University of California-Merced, Merced, 95343, CA, USA. Electronic address: wchin2@ucmerced.edu.

PMID: 29929165
DOI: 10.1016/j.colsurfb.2018.06.011

Abstract

The highly prevalent and virulent disease in the Western Hemisphere Coccidioidomycosis, also known as Valley Fever, can cause serious illness such as severe pneumonia with respiratory failure. It can also take on a disseminated form where the infection spreads throughout the body. Thus, a serious impetus exists to develop effective detection of the disease that can also operate in a rapid and high-throughput fashion. Here, we report the assembly of a highly sensitive biosensor using reduced graphene oxide (rGO) with Coccidioides(cocci) antibodies as the target analytes. The facile design made possible by the scalable microcontact printing (μCP) surface patterning technique which enables rapid, ultrasensitive detection. It provides a wide linear range and sub picomolar (2.5 pg/ml) detection, while also delivering high selectivity and reproducibility. This work demonstrates an important advancement in the development of a sensitive label-free rGO biosensor for Coccidioidomycosis detection. This result also provides the potential application of direct pathogen diagnosis for the future biosensor development.

Keywords: Coccidioidomycosis (cocci); High-throughput; Microcontact printing (μCP); Reduced graphene oxide (rGO); Sensor.

MeSH terms

Antibodies, Fungal
Biosensing Techniques*
Coccidioidomycosis / diagnosis*
Coccidioidomycosis / microbiology*
Graphite / chemistry*
High-Throughput Screening Assays / methods*
Humans
Oxides / chemistry*
Particle Size
Printing*
Surface Properties

Substances

Antibodies, Fungal
Oxides
Graphite