Structure of the O-specific polysaccharide from Azospirillum fermentarium CC-LY743T

Elena N Sigida; Yuliya P Fedonenko; Alexander S Shashkov; Svetlana A Konnova; Vladimir V Ignatov

doi:10.1016/j.carres.2018.06.003

Structure of the O-specific polysaccharide from Azospirillum fermentarium CC-LY743^T

Carbohydr Res. 2018 Jul 30:465:40-43. doi: 10.1016/j.carres.2018.06.003. Epub 2018 Jun 6.

Authors

Elena N Sigida¹, Yuliya P Fedonenko², Alexander S Shashkov³, Svetlana A Konnova⁴, Vladimir V Ignatov²

Affiliations

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia. Electronic address: si_elena@mail.ru.
² Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia.
³ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospekt, Moscow, 119991, Russia.
⁴ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia; N. G. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov, 410012, Russia.

PMID: 29929051
DOI: 10.1016/j.carres.2018.06.003

Abstract

O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of nitrogen-fixing bacterium Azospirillum fermentarium CC-LY743^T (IBPPM 578) and was studied by sugar analysis along with ¹H and ¹³C NMR spectroscopy, including ¹H,¹H COSY, TOCSY, ROESY, and ¹H,¹³C HSQC and HMBC experiments. The polysaccharide was found to be linear and to consist of alterating α-l-fucose and α-d-mannose residues in tetrasaccharide repeating units of the following structure: →2)-α-D-Manp-(1 → 3)-α-L-Fucp-(1 → 3)-α-D-Manp-(1 → 3)-α-L-Fucp-(1→.

Keywords: Azospirillum fermentarium; Bacterial polysaccharide structure; Lipopolysaccharide.

MeSH terms

Azospirillum / chemistry*
Carbohydrate Conformation
Nuclear Magnetic Resonance, Biomolecular
Polysaccharides, Bacterial / chemistry*

Substances

Polysaccharides, Bacterial