Abstract
Store-operated channels activated in response to intracellular calcium store depletion represent the main pathway of calcium entry from the extracellular space in nonelectroexcitable cells. Adapter proteins organize the components of this system into integral complex. We studied the influence of adapter proteins of the Homer family on endogenous store-operated calcium Imin channels in A431 cells. Monomeric Homer 1a proteins increase activity of Imin channels, but did not modulate their electrophysiological properties. Recombinant Homer 1c protein did not block the induced calcium currents.
Keywords:
Homer; IP3R; STIM; single channels; store-operated calcium influx.
MeSH terms
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Action Potentials / drug effects
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Calcium / metabolism*
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Calcium Channel Agonists / metabolism
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Calcium Channel Agonists / pharmacology
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Calcium Channels / drug effects
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Calcium Channels / metabolism*
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Calcium Channels / physiology
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Calcium Signaling / drug effects
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Cytoplasm / metabolism
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Electrophysiological Phenomena / drug effects
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Homer Scaffolding Proteins / pharmacology
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Homer Scaffolding Proteins / physiology*
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Humans
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Ion Channel Gating / drug effects
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Patch-Clamp Techniques
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Protein Multimerization / physiology
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Recombinant Proteins / pharmacology
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Tumor Cells, Cultured
Substances
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Calcium Channel Agonists
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Calcium Channels
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Homer Scaffolding Proteins
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Recombinant Proteins
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Calcium