GREEN FLUORESCENT SEED, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds

Methods Mol Biol. 2018:1789:1-7. doi: 10.1007/978-1-4939-7856-4_1.

Abstract

Vacuolar trafficking plays a vital role in plant growth and development. In this chapter, we describe a powerful technique for the evaluation of vacuolar protein trafficking, which is designated as GREEN FLUORESCENT SEED. Based on vacuole-targeted green fluorescent protein in Arabidopsis seeds, this method enables the nondestructive isolation of mutant seeds defective in vacuolar trafficking and their visual characterization.

Keywords: Arabidopsis thaliana; GREEN FLUORESCENT SEED (GFS); Green fluorescent protein (GFP); Vacuolar sorting receptor 1 (VSR1); Vacuolar trafficking; Vacuole.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism*
  • Microscopy, Confocal / methods
  • Microscopy, Fluorescence / methods
  • Mutagenesis
  • Protein Transport
  • Seeds / genetics
  • Seeds / metabolism*
  • Vacuoles / genetics
  • Vacuoles / metabolism*
  • Vesicular Transport Proteins / genetics
  • Vesicular Transport Proteins / metabolism*

Substances

  • Arabidopsis Proteins
  • Vesicular Transport Proteins
  • Green Fluorescent Proteins