Anti-proliferative applications of laccase immobilized on super-magnetic chitosan-functionalized halloysite nanotubes

Abstract

Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe₃O₄) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe₃O₄-HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe₃O₄-HNTs-CS (termed as Fe₃O₄-HNTs-CS-Lac). Then free laccase and Fe₃O₄-HNTs-CS-Lac were evaluated for anti-proliferative properties against cancer cell lines of liver (HepG2), lung (H460), cervix (Hela) and stomach (AGS). Laccase and Fe₃O₄-HNTs-CS-Lac gave significant cytotoxicity against all studied cancer cell lines. Moreover, the apoptosis analysis and FE-SEM morphology observations of cells support the anti-proliferative potential of laccase immobilized on Fe₃O₄-HNTs-CS. Therefore, investigated Fe₃O₄-HNTs-CS-Lac is natural and super-magnetic nano-biocatalyst, having the significant anti-proliferative potential and furthermore, Fe₃O₄-HNTs-CS can be used as efficient and specific delivery system for other anti-cancer enzymes.

Keywords: Anti-proliferative laccase; Halloysite nanotubes; Super-magnetic drug carrier; Trametes versicolor laccase.