Crystallization and preliminary x-ray investigation of the regulatory subunit of cAMP-dependent protein kinase

J Biol Chem. 1985 Aug 5;260(16):9380-1.

Abstract

Crystals of type I cAMP-dependent protein kinase regulatory subunit have been grown from solutions of ammonium sulfate. The crystals are square bipyramids, space group P4(1)2(1)2 (P4(3)2(1)2), with a = b = 106.9 +/- 0.6 A and c = 212.4 +/- 1.0 A. There are two dimers of the regulatory subunit/crystallographic asymmetric unit. The crystals are stable for 3-4 days in the x-ray beam and diffract to at least 3.5-A resolution.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Crystallization
  • Cyclic AMP / metabolism
  • Muscles / enzymology
  • Protein Conformation
  • Protein Kinases / isolation & purification*
  • Protein Kinases / metabolism
  • X-Ray Diffraction / methods

Substances

  • Cyclic AMP
  • Protein Kinases