In vitro interaction and biocompatibility of titanate nanotubes with microglial cells

Toxicol Appl Pharmacol. 2018 Aug 15:353:74-86. doi: 10.1016/j.taap.2018.06.013. Epub 2018 Jun 13.

Abstract

Titanate nanotubes (TiONts) are promising agents for biomedical applications. Microglial activation and associated oxidative burst are major challenges in drug delivery applications across the brain. Here, TiONts were designed for drug delivery systems by functionalizing them with (3-aminopropyl) triethoxysilane (APTES), their interactions and biocompatibility were studied in vitro using murine microglial BV-2 cells. TiONts-APTES exposure resulted in increased ROS production and transient mitochondrial hyperpolarization. However, there was no indication of microglial proliferation in BV-2 cells as suggested by cell cycle analysis and morphology evaluation. The endocytosis as well as passive diffusion mediated TiONts-APTES internalization were proved by transmission electron microscopy (TEM) with and without amiloride, an endocytosis inhibiting agent. In addition, the TiONts-APTES exhibited good biocompatibility on microglial BV-2 cells as revealed by the plasma membrane integrity, lysosmal membrane integrity, morphology and viability analysis.

Keywords: Apoptosis; Lysosomal integrity; Microglial activation; Mitochondrial hyperpolarisation; Reactive oxygen species; Titanate nanotubes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocompatible Materials / toxicity*
  • Cell Line
  • Cell Membrane / drug effects
  • Cell Proliferation / drug effects
  • Endocytosis / drug effects
  • Humans
  • Lysosomes / drug effects
  • Materials Testing*
  • Membrane Potential, Mitochondrial / drug effects
  • Microglia / drug effects*
  • Nanotubes / toxicity*
  • Reactive Oxygen Species
  • Respiratory Burst / drug effects
  • Titanium / toxicity*

Substances

  • Biocompatible Materials
  • Reactive Oxygen Species
  • titanium dioxide
  • Titanium