Eggerthella timonensis sp. nov, a new species isolated from the stool sample of a pygmy female

Microbiologyopen. 2018 Oct;7(5):e00575. doi: 10.1002/mbo3.575. Epub 2018 Jun 13.

Abstract

Eggerthella timonensis strain Marseille-P3135 is a new bacterial species, isolated from the stool sample of a healthy 8-year-old pygmy female. This strain (LT598568) showed a 16S rRNA sequence similarity of 96.95% with its phylogenetically closest species with standing in nomenclature Eggerthella lenta strain DSM 2243 (AF292375). This bacterium is a nonspore forming, Gram-positive, nonmotile rod with catalase but no oxidase activity. Its genome is 3,916,897 bp long with 65.17 mol% of G + C content. Of the 3,371 predicted genes, 57 were RNAs and 3,314 were protein-coding genes. Here, we report the main phenotypic, biochemical, and genotypic characteristics of E. timonensis strain Marseille-P3135 (=CSUR P3135, =CCUG 70327); ti.mo.nen'sis, N.L. masc. adj., with timonensis referring to La Timone, which is the name of the hospital in Marseille (France) where this work was performed). Strain is a nonmotile Gram-positive rod, unable to sporulate, oxidase negative, and catalase positive. It grows under anaerobic conditions between 25°C and 42°C but optimally at 37°C.

Keywords: Eggerthella timonensis; culturomics; genome; new species; pygmy; taxonogenomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / classification*
  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification*
  • Anaerobiosis
  • Bacterial Typing Techniques
  • Base Composition
  • Catalase / analysis
  • Child
  • Cluster Analysis
  • Congo
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Feces / microbiology*
  • Female
  • Genotype
  • Humans
  • Locomotion
  • Mass Spectrometry
  • Microscopy, Electron, Transmission
  • Oxidoreductases / analysis
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Temperature
  • Whole Genome Sequencing

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Oxidoreductases
  • Catalase

Associated data

  • GENBANK/LT598568