Structural analysis and antioxidant activities of neutral polysaccharide isolated from Epimedium koreanum Nakai

Bo Li; Ning Zhang; Dong Xue Wang; Lili Jiao; Ying Tan; Jing Wang; Hui Li; Wei Wu; Da Cheng Jiang

doi:10.1016/j.carbpol.2018.05.037

Structural analysis and antioxidant activities of neutral polysaccharide isolated from Epimedium koreanum Nakai

Carbohydr Polym. 2018 Sep 15:196:246-253. doi: 10.1016/j.carbpol.2018.05.037. Epub 2018 May 17.

Authors

Bo Li¹, Ning Zhang¹, Dong Xue Wang², Lili Jiao², Ying Tan¹, Jing Wang¹, Hui Li², Wei Wu³, Da Cheng Jiang⁴

Affiliations

¹ School of Pharmaceutlcal Sciences, Changchun University of Chinese Medicine, Changchun 130024, China.
² Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
³ Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: wuwei_ccucm@126.com.
⁴ School of Pharmaceutlcal Sciences, Changchun University of Chinese Medicine, Changchun 130024, China. Electronic address: 13944103315@163.com.

PMID: 29891293
DOI: 10.1016/j.carbpol.2018.05.037

Abstract

The structure and antioxidant activity of a neutral polysaccharide (named as EFPN-1) from Epimedium koreanum Nakai were studied. Based on the results from high performance gel permeation chromatography, methylation GC-MS and 1D/2D nuclear magnetic resonance spectroscopy, EFPN-1 was a branched polysaccharide with a molecular weight of 21.9 kD and containing over 60% of glucose. Its backbone was composed of (1 → 4)-β-d-Glcp and (1 → 3)-β-d-Galp, branched partially at O-6 of both residues, with a degree of branching of 40.4% and terminated mainly with Ara and Gal, as well as trace amount of Gal. Antioxidant tests showed that EFPN-1 exhibited a strong capacity on DPPH and hydroxyl radical scavenging, ferrous ion chelation and reducing antioxidant powder. EFPN-1 has potential to be developed as a natural antioxidant.

Keywords: Antioxidant activity; Epimedium koreanum Nakai; Neutral polysaccharides; Structure.