In vitro effects of brominated flame retardants, selected metals and their mixtures on ethoxyresorufin-O-deethylase activity in Mossambica tilapia liver

Abstract

The in vitro effects of individual brominated flame retardants (BFRs), selected metals, and their binary mixtures on ethoxyresorufin-O-deethylase (EROD) activity were evaluated using a plate-reader method. The BFRs, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), decabromodiphenyl oxide (BDE-209), hexabromocyclododecane (HBCD), and tetrabromobisphenol A (TBBPA), were tested at doses ranging from 0.1 ng/L to 100 µg/L. Selected metals (Cu²⁺, Cd²⁺, Hg²⁺, and Zn²⁺) were screened at doses of 0.1 mg/L to 50 mg/L. The activity of EROD was significantly induced by TBBPA, BDE-209, and Zn²⁺, while HBCD, Cu²⁺, Cd²⁺, and Hg²⁺ decreased EROD activity. Moreover, following exposure to binary mixtures of metals and BFRs, the EROD activity dose-response curves were similar to those of the metals alone, indicating that EROD activity was governed by the metals.

Keywords: Brominated flame retardants (BFRs); EROD activity; In vitro; Metals; Mixtures; Toxic effect.