Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ

Methods Mol Biol. 2018:1820:147-155. doi: 10.1007/978-1-4939-8609-5_12.

Abstract

Electrophysiological recording and optical imaging enable the characterization of membrane and odorant response properties of olfactory sensory neurons (OSNs) in the nasal neuroepithelium. Here we describe a method to record the responses of mammalian OSNs to odorant stimulations in an ex vivo preparation of intact olfactory epithelium. The responses of individual OSNs with defined odorant receptor types can be monitored via patch-clamp recording or calcium imaging.

Keywords: Calcium imaging; Electrophysiology; GCaMP6; Gene targeting; Olfactory sensory neurons; Patch-clamp; Transduction.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Calcium Signaling / physiology*
  • Membrane Potentials / physiology*
  • Mice
  • Molecular Imaging / methods*
  • Olfactory Receptor Neurons / cytology
  • Olfactory Receptor Neurons / metabolism*
  • Patch-Clamp Techniques / methods