NMDA and GABA receptor presence in rat heart mitochondria

Semen V Nesterov; Yulia A Skorobogatova; Alisa A Panteleeva; Lyubov L Pavlik; Irina B Mikheeva; Lev S Yaguzhinsky; Yaroslav R Nartsissov

doi:10.1016/j.cbi.2018.06.004

NMDA and GABA receptor presence in rat heart mitochondria

Chem Biol Interact. 2018 Aug 1:291:40-46. doi: 10.1016/j.cbi.2018.06.004. Epub 2018 Jun 6.

Authors

Semen V Nesterov¹, Yulia A Skorobogatova², Alisa A Panteleeva³, Lyubov L Pavlik⁴, Irina B Mikheeva⁵, Lev S Yaguzhinsky⁶, Yaroslav R Nartsissov⁷

Affiliations

¹ Moscow Institute of Physics and Technology, Institutskiy pereulok, 9, Dolgoprudny, 141700, Russia. Electronic address: semen.v.nesterov@phystech.edu.
² Moscow Institute of Physics and Technology, Institutskiy pereulok, 9, Dolgoprudny, 141700, Russia. Electronic address: skorobogatova@phystech.edu.
³ Belozersky Research Institute for Physico Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia. Electronic address: jalice@ya.ru.
⁴ Institute of Theoretical and Experimental Biophisics Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. Electronic address: pavlikl@mail.ru.
⁵ Institute of Theoretical and Experimental Biophisics Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. Electronic address: mikheirina@yandex.ru.
⁶ Institute of Cytochemistry and Molecular Pharmacology, 6-th Radialnaya str. 24-14, Moscow, 115404, Russia; Belozersky Research Institute for Physico Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. Electronic address: yag@genebee.msu.su.
⁷ Institute of Cytochemistry and Molecular Pharmacology, 6-th Radialnaya str. 24-14, Moscow, 115404, Russia. Electronic address: yarosl@biotic.dol.ru.

PMID: 29883723
DOI: 10.1016/j.cbi.2018.06.004

Abstract

The purpose of this study is to demonstrate the presence of three more receptors in mitochondria. Two N-methyl-d-aspartate receptor (NMDAR) subunits (NR1 and NR2B) are found by protein immunoblot and immunogold labeling in mitochondria fraction isolated from rat heart. These data allow supposing NMDAR presence and functioning in the inner mitochondrial membrane. There are no signs of receptor presence obtained in heart tissue lysate, that indicates the receptor localization exactly in mitochondria. The possible receptor functions discussed are its participation in calcium transport and in excitation-metabolism coupling. Besides, preliminary evidence is obtained of GABA_A and GABA_B receptors presence in heart mitochondria. One can surmise their role in metabolism regulation and their possible co-operation with NMDAR just as in the nervous system.

Keywords: GABA receptor; Heart metabolism; Mitochondria; NMDA receptor.

MeSH terms

Animals
Blotting, Western
Mitochondria, Heart / metabolism*
Mitochondria, Heart / ultrastructure
Mitochondrial Membranes / metabolism
Protein Subunits / metabolism
Rats, Wistar
Receptors, GABA / metabolism*
Receptors, N-Methyl-D-Aspartate / metabolism*

Substances

Protein Subunits
Receptors, GABA
Receptors, N-Methyl-D-Aspartate